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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants were described but thorough nucleotide sequence info is not really but available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide usually is made up of the epitope regarded from the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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